Lens-less microscopy / holographic multi-angle on chip microscopy for biomedicine

1. Gradov O.V., Notchenko A.V. (2011). Topological laser speckle analyzer of differentiation and proliferation activity during morphogenesis in cell cultures. Morphologia, 5:10-19 (in Ukrainian).
2. Gradov O.V., Notchenko A.V. (2012). Hemocytometer-based simple morphometric lab-on-a-chip with RF-identification and translation of cell culture histochemical monitoring data. Journal of Radio Electronics, 2: http://jre.cplire.ru/jre/feb12/5/abstract_e.html (in Russian).
3. Notchenko A.V., Gradov O.V. (2013). Elementary morphometric labs-on-a-chip based on hemocytometric chambers with radiofrequency culture identification and relay of spectrozonal histochemical monitoring. Visualization, Image Processing and Computation in Biomedicine, 2. DOI: 10.1615/VisualizImageProcComputatBiomed.2013005968 [Translated from the «Journal of Radio Electronics», 2012].
4. Notchenko A.V., Gradov O.V. (2013). A five-axis arm-manipulator laser system and an algorithm for digital processing of output data for recording and morpho-topological identification of cells and tissue structure. Visualization, Image Processing and Computation in Biomedicine: 2. DOI: 10.1615/VisualizImageProcComputatBiomed.2013005967 [Translated from the «Journal of Radio Electronics», 2012].
5. Alexandrov P.L., Notchenko A.V., Gradova M.A., Gradov O.V. (2015). Simultaneous in situdetection of the optical fluorescence, fluorescence recovery kinetics after photobleaching and membrane ion flux on the electrophysiological lab-on-a-chip. American Journal of Optics and Photonics, 3(5):118–122.
6. Gradov O.V., Jablokov A.G. (2016). Novel morphometrics-on-a-chip: CCD- or CMOS-lab-on-achip based on discrete converters of different physical and chemical parameters of histological samples into the optical signals with positional sensitivity for morphometry of non-optical patterns. Journal of Biomedical Technologies, (2):1–29.
7. Gradov O.V., Yablokov A.G. (2017). Morphophysiological morphofunctional analysis for lymphological problems in microfluidic chips. Translational Medicine, (S3):14 (in Russian).
8. Gradov O.V., Jablokov A.G. (2017). Multiparametric lab-on-a-chip with miltiple biophysical signal converters as a novel tool for experimental stem cell biology and control equipment for hematopoetic stem cell transplantation. Cellular Therapy and Transplantation, 6(3): 41–42.
9. Gradov O.V., Yablokov A.G. (2017). Multiparametric lab-on-a-chip as a tool for experimental regenerative medicine and synthetic morphogenesis. Genes and Cells, 12(3):74 (in Russian).
10. Gradov O.V., Nasirov P.A., Scrynnic A.A., Jablokov A.G. (2017). A simple device for microinjections, manipulations and measurements using an electromorphological chip under microinterferometric control of the interface and membrane processes at the thickness range of 5-1000 nm at different angles. Morphologia, 11(4):7-17 (in Ukrainian).
11. Nasirov F.A., Jablokov A.G., Gradov O.V. (2018). Registration of the preparation of seminiferous tubules by methods of lensless laser projection (holographic) microscopy on a chip and near-field microscopy with spectral zonal decomposition of image channels. Genes and Cells, 13 (Suppl. II): 85–86 (in Russian).
12. Gradov O.V., Nasirov P.A., Jablokov A.G. (2018). Lensless on-chip-hemocytometry with secondary processing of cell images in the framework of an unconventional photometric model. Photonics Russia, 12(7): 716–729.
13. Gradov O.V., Krukowskikh V.V., Nasirov P.A., Jablokov A.G. (2018). Multi-axis goniometric 3Dvisualization of vector diagrams of optical characteristics of dispersed and biological structures on a chip using different laser scanning regimes and trajectories. Photonics Russia, 4(72):448–458.

Lens-less microscopy for food qualimetry

14. Gradov O.V. Lensless Holographic Osmometer. Patent # WO 2022/098264 (12 May 2022). — PCT/RU2021/050357 (27 October 2021).
15. Gradov O.V. (2021). Lens-less multispectral and hyperspectral microscopy for food qualimetry. In International Conference Food Biotech 2021, Abstract Booklet, Section “Food Processing and Process Control”, 23-24 August, 2021, St. Petersburg, ITMO Univeristy1.
16. Orekhov T., Gradov O. (2021). Digital spectrozonal and multispectral lens-less devices with spectrophotometric temperature calibration GUIs for dairy farming and qualimetry of diary products. Lecture Notes in Networks and Systems, 228:300–324.
17. Orekhov F.K., Gradov O.V. (2016). Comparative labs-on-a-chip for dairy product analysis with automatic calibration using spectrophotometric or colorimetric temperature and tuple chemometric analyte systematization. J. Adv. Dairy Res., 4(Conf. Suppl. 2):24.

Lens-less microscopy for soil microbiology / soil science and foundation monitoring

18. Gradov O.V. (2012). Digital lab-on-a-chip as analog of soil chambers and Rossi-Cholodny slides. In VII Int. Symp. «Cooperation in Biotechnology, Agriculture, Forestry, Fisheries and Food in the 7th Framework Programme», A. Bach Institute of Biochemistry, Moscow, p. 17-18.
19. Orekhov F., Gradov O. (2022). Tensoresistor-based microfluidics and telemetric strain-gauge lensless detectors as specialized labs-on-a-chip for soil mechanics and foundation monitoring. Lecture Notes in Civil Engineering, 180:371–380.
20. Orekhov F., Gradov O. (2022). Automated soil microbiology using lens-less and LDI MS imaging with buried slides. Smart Innovation, Systems and Technologies, 247:471–479.
21. Gradov O.V. (2022). Towards comprehensive environmental studies of the physiology and biochemistry of microorganisms based on mass spectrometric decoding of coupled inter-specific and populational processes in the frameworks of the geochemical ecology on a chip. In 3rd International Webinar on Mass Spectrometry & Analytical Techniques (April 18-19, 2022, Greenville, USA, virtual event), p. 14–15.
22. Orekhov F., Gradov O. (2023) Target Chip Based Single-Cell Biotyping and Telemetric Bioluminescence Lensless Microscopy of the Buried Sandwich-Slides as a Novel Way for Measurement, Mapping and Molecular Imaging of Biodegradation / Biofouling of Plastic Surfaces in Real Soils. Advances in Transdisciplinary Engineering, 38:417-425.

Microfuidics/optofluidics for experimental physical chemistry

23. Gradov O.V., Gradova M.A. (2018). Soft matter reaction-diffusion and ferrofluid patterns as dynamic microchannels for optoelectronic lab-on-a-chip with the field-controlled geometry / topology (Seminar Paper). Comp. Nanotech., (4):75–77.
24. Adamovich E.D., Gradov O.V., Nasirov P.A. (2018). Liquid-metal microfluidics with morphometric correlation-spectral control of the medium. INTERMATIC-2018 Conference. Fundamental Problems of Radioelectronic Instrumentation, 18(3):650–653 (in Russian).
25. Gradov O.V. (2016). Multiparametric lab-on-a-chip for biochemical and physico-chemical measurements with reference to the coordinates of the mapped sample. In Proc. Conference and Exhibition «Scientific Instrumentation — current state and prospects», p. 183–185 (in Russian).

There were several R&D projects on this topic held in 2016-2018 by O.V. Gradov as a single PI:

RFBR project 16-32-00914 «Development of the novel physical methods for complex biomedical diagnostics based on position-sensitive mapping with angular resolution at tissue and cellular levels using analytical labs-on-a-chip» (holographic lens-less imaging) — 2016-2017 (total funding amount ≈ 9,000 euro at the current euro exchange rate in the Russian Federation);

FASIE project 0019125 «Development of labs-on-a-chip for personalized diagnostics» (multispectral lens-less imaging) — 2016-2018 (total funding amount ≈ 4,000 euro at the current euro exchange rate in the Russian Federation).

Less than 20% of the results obtained have been published, since the group received funding from the end of 2016 up to the middle of 2018, and in November 2018 it was already expelled from the institute during preparing it for reorganization.

The works published were appreciated by the specialists and found a wide audience:
a) O.V. Gradov is a laureate of the youth scientific and innovative competition of the Foundation for Assistance to the Development of Small Forms of Scientific and Technical Enterprises in the Russian Academy of Sciences (2015).
b) The results obtained during the implementation of the RFBR Project were recommended by the experts for publication in the annual scientific government report (2018).
c) A paper on the lensless system for multi-axis 3D visualization of vector diagrams of the optical characteristics of colloidal and biological structures on a chip at various trajectories and laser scanning modes was at the top of the Top 10 rating of the «Technosfera» publishing house in 2018.
d) Papers on the lensless scanning scalpel (for analysis of the sectioned tissues during microtomy and surgery) were included in Top 9 Arxiv Papers Today in Subcellular Processes (2018).